Download Adhesion of Microbial Pathogens by John N. Abelson, Melvin I. Simon, Ron J. Doyle, Itzhak Ofek PDF

By John N. Abelson, Melvin I. Simon, Ron J. Doyle, Itzhak Ofek

The severely acclaimed laboratory typical for 40 years, Methods in Enzymology is likely one of the such a lot hugely revered guides within the box of biochemistry. due to the fact that 1955, every one quantity has been eagerlyawaited, usually consulted, and praised via researchers and reviewers alike. greater than 250 volumes were released (all of them nonetheless in print) and masses of the cloth is appropriate even today--truly a vital booklet for researchers in all fields of existence sciences.

Key Features
* techniques all for learning adhesion
* Genetic manipulation of adhesions
* Adhesion and receptor isolation and characterization
* Distinguishes among adhesion and invasion
* Assays for adhesion
* Kinetic and epidemiological concerns of adhesion and an infection

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Mizutani, L. J. Altass, S. M. Watt, M. C. Jacob, P. van den Elsen, C. Terhorst, and M. F. Greaves, Blood 68, llf)l (1986). , iron) might adhere to phagocytes quite differently. As mentioned above, the degree of neutrophil priming or activation and of monocyte or macrophage differentiation can dramatically affect bacterial adhesion. 5 mg/ml cytochalasin B or D in dimethyl sulfoxide (DMSO); the stock should be kept in small aliquots, frozen at - 2 0 °, and thawed and used only once Cytocentrifuge (Cytospin, Shandon Southern Instruments, Sewickley, PA) Wright stain, glass slides, light microscope, immersion oil Methods.

METHODS IN ENZYMOLOGY, VOL. 253 Copyright © 1995 by Academic Press, Inc. All rights of reproduction in any form reserved. [2] ASSOCIATION OF BACTERIA WITH HUMAN PHAGOCYTES 13 Before presenting specific assays, a short introduction to the interacting components and their general characteristics and interactions is required. The assays, which are generally rather simple to set up and perform, consist of incubating bacteria and phagocytes, sometimes with serum or other body fluids, and then quantifying adhesion.

MicrobioL 7, 111 (1993). [2] ASSOCIATION OF BACTERIA WITH HUMAN PHAGOCYTES 21 TABLE I HUMAN NEUTROPHIL-, MONOCYTE-, AND MACROPHAGE-LIKE CELL LINES Cell line Derived from HL-60 Promyelocytic leukemia K-562 Chronic myelogenous leukemia; erythroleukemia line KG-1 Acute myelogenous leukemia THP-1 Acute monocytic leukemia Histiocytic lymphoma U-937 Description Undifferentiated, nonadherent; differentiates to myelocytic or monocytic lineage in presence of various stimuli; very commonly used; well-characterized Undifferentiated, nonadherent; differentiates to myelocytic or monocytic lineage in presence of various stimuli; not commonly used or well-characterized for phagocytosis studies Undifferentiated, nonadherent; differentiates to myelocytic or monocytic lineage in presence of various stimuli; not commonly used or well-characterized for phagocytosis studies Monocyte- and macrophage-like characteristics Undifferentiated, nonadherent; differentiates to myelocytic or monocytie lineage in presence of various stimuli; commonly used; well-characterized ATCC number Ref.

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