By Mitsuo Satoh, Shigeru Iida (auth.), Sanetaka Shirahata, Koji Ikura, Masaya Nagao, Akira Ichikawa, Kiichiro Teruya (eds.)
Animal mobile expertise is a growing to be self-discipline of phone biology which goals not just to appreciate constructions, capabilities and behaviors of differentiated animal cells, but additionally to examine their talents for use for commercial and scientific reasons. The objective of animal cellphone expertise contains the clonal enlargement of differentiated cells, the optimization in their tradition stipulations, modulation in their skill to supply proteins of scientific and pharmaceutical importantance, and the appliance of animal cells to gene treatment, man made organs and the construction of practical meals. This quantity offers the readers an entire assessment of the current state of the art and should be precious for these operating in both educational environments or within the biotechnology and pharmaceutical sectors, relatively phone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and all different disciplines regarding animal mobile culture.
Read Online or Download Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006 PDF
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Extra info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 19th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), Kyoto, Japan, September 25-28, 2006
After mounting the strips in the electrofocusing chamber unit (Anatech, Japan), the proteins were separated according to their isoelectric points. The details of the steps are shown in Table 1. 8, 6 M urea, 30% glycerol, and 4% SDS, with addition of 10 mg/ml DTT (solution A) or 90 mg/ml iodoacetamide instead of DTT (solution B). 5% SDSpolyacrylamide gels with the Cool Phorestar (Anatech). The immobiline Dry-Strips were sealed on the top of the gels using a sealing 1% agarose solution. The proteins were separated by 20–40 mA/slab, according to their molecular weight, until the BPB dye approached the bottom of the gel [3, 4].
Yamanaka, H. Yamada, N. Fuwa, M. Nomura (1998) Biosci. Biotechnol. , 62, 145–147. 5. F. Makishima, S. Terada, T. Mikami and E. Suzuki (1992) Cytotechnology, 10, 15–23. Molecular Biological Analysis of Mitogenic and Anti-Apoptotic Mechanisms of Sericin Takuya Saito, Akiko Ogawa, Masahiro Sasaki, Hideyuki Yamada, and Satoshi Terada Abstract We have previously reported that the silk protein sericin has mitogenic activity in all cells tested, as well as preventing cell death from several stimuli such as hyperthermia.
Mikami and E. Suzuki (1992) Cytotechnology, 10, 15–23. Molecular Biological Analysis of Mitogenic and Anti-Apoptotic Mechanisms of Sericin Takuya Saito, Akiko Ogawa, Masahiro Sasaki, Hideyuki Yamada, and Satoshi Terada Abstract We have previously reported that the silk protein sericin has mitogenic activity in all cells tested, as well as preventing cell death from several stimuli such as hyperthermia. The mechanism of how sericin induces cell proliferation and suppresses cell death has not been established.