By Albert Yen Chang (auth.), Kouji Ikura, Masaya Nagao, Seiji Masuda, Ryuzo Sasaki (eds.)
Animal telephone know-how is a starting to be self-discipline of telephone biology which goals not just to appreciate buildings, features and behaviors of differentiated animal cells but additionally to envision their talents for use in commercial and clinical reasons. The target of animal mobile expertise contains accomplishments of clonal growth of differentiated cells with beneficial skill, optimization in their tradition stipulations, modulation in their skill to supply medically and pharmaceutically, very important proteins, and the appliance of animal cells to gene remedy and synthetic organs. This quantity offers the readers an entire evaluate of current state-of-the-art in Japan. The complaints may be worthy for telephone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and different disciplines regarding animal mobilephone tradition, operating in both educational environments or in biotechnology and pharmaceutical industries.
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Additional info for Animal Cell Technology: Challenges for the 21st Century: Proceedings of the joint international meeting of the Japanese Association for Animal Cell Technology (JAACT) and the European Society for Animal Cell Technology (ESACT) 1998, Kyoto, Japan
This means that batch cultures of CHO320 cells. at least under the typical conditions in this laboratory. were close to a steady state at any one particular time. Since glucose flux cannot be determined easily from the concentration data. a set of flow cytometric experiments were arranged to determine the intensity of mitochondrial membrane potential at different dilution rates. The results arc depicted in Figure 3, Although the relationship between the curve for fluorescence intensity by rhodamine123 and mitochondrial membrane potential is complex.
The culture period could be separated into two phases: the cell growth phase, and the cell maintenance phase during which a high cell density was maintained. The specific growth rate during the cell growth phase increased (Fig. l), and cell decreasing rate during the cell maintenance phase decreased (Fig. 2) with increase in the concentration of serum in the medium, while the specific glucose consumption rate and the conversion rate of glucose to lactate did not vary in both phases. The ratio of ammonia production to glutamine consumption during the cell growth phase increased as the concentration of serum in the medium increased although the specific glutamine 29 30 consumption rate decreased.
Abstract CHO cells were successfully cultured on a novel type of microcarriers (ImmobaSil FS) in the bioreactors. Various factors influencing cell attachment, including serum concentration, cell to microcarrier ratio, agitation speed and agitation profile were investigated and the results were compared with those obtained using CultiSpher-G, and glass beads. It was found that the optimum serum concentration, cell-to- carrier ratio and agitation speed for the batch reactor with a working volume of 400 ml during the initial attachment period were 10% (vh), 12x103:l and 60 rpm, respectively.