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Low molecular metabolites permeate through the membrane according to the concentration gradient from culture to dialysis chamber. During dialysis phase a buffer is used to exchange the medium in the dialysis chamber to maintain the gradient in metabolite concentration and to remove inhibiting metabolites. This concept is realised in the Membrane Dialysis Reactor (Bioengineering, CH) shown in figure 2. Figure 2: Membrane Dialysis Reactor 5. 5-fold in viable cell concentration Xv (Fig. 3) and 3-fold in antibody concentration CMAb (Fig.
ZHANG & M. uk/ChemEng/actg/ac tghomehtm ) Keywords: Cell attatchment, CHO cells, Porous microcarriers 1. Abstract CHO cells were successfully cultured on a novel type of microcarriers (ImmobaSil FS) in the bioreactors. Various factors influencing cell attachment, including serum concentration, cell to microcarrier ratio, agitation speed and agitation profile were investigated and the results were compared with those obtained using CultiSpher-G, and glass beads. It was found that the optimum serum concentration, cell-to- carrier ratio and agitation speed for the batch reactor with a working volume of 400 ml during the initial attachment period were 10% (vh), 12x103:l and 60 rpm, respectively.
0x105 cells ml-1and 60 rpm of agitation were used. 0x105 cells ml-1, RPMT 1640 supplemented with 10% FBS and different agitation speeds ranged from 20 to 120 rpm were used. 0x105 cells ml-l and 60 rpm of agitation were used. The variation of the number of cells attaching on ImmobaSil FS microcarriers was quantified by the MTT method. 40 carriers were transferred into a 96-well plate. Each well contained a single microcarrier, in which 100 µl MTT solution (5mg ml-l) was added. The optical intensity of each sample was measured by a spectrophotometer (Pharmacia, Sweden).